By Marc De Ley

A set of biochemical, mobile, and molecular thoughts for unraveling and quantifying the occasions happening among the preliminary touch of a cytokine on the membrane receptor and the eventual activation of gene transcription. The ideas used comprise the new release of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights contain RT-PCR of small quantities of mRNA, in situ hybridization, biosensor research, dimension of organic actions and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization.

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