By K H Andy Choo
Thirty-one precise, functional chapters many via the originators themselves current easy-to-follow protocols of in situ hybridization innovations for mapping DNA sequences onto chromosomes, quantitation/localization of RNA in tissues, and detection of virus nucleic acids. The equipment serve molecular biologists in so much parts of simple and utilized learn, and in each kind of organism starting from viruses to Drosophila to people. The professional members offer step by step directions for the training of probes for DNA, teleomeric repeats, cDNA sequences, small man made oligomers, entire cosmids, mega-size YACs, and chromosome-specific paints from somatic mobilephone hybrids and flow-sorted chromosomes. additionally they current radioactive and nonradioactive probe labeling tactics with using nick translation, random priming, multicolor labeling, PRINS labeling of DNA and RNA, and PCR in situ hybridization. colour illustrations increase the textual content. Troubleshooting information, other ways of doing issues, and informative factors of why sure steps are performed -- aids now not often present in usual magazine recipes -- warrantly an important distinction within the final result of experiments.
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